Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in studies of phage biology

Article Abstract:

A procedure for construction and isolation of specifically designed mutations of the temperate lactococcal phage TP901-1 was designed. This technique was based on homologous recombination between the wild-type phage and a plasmid consisting of a phage DNA fragment having the desired mutation. It was shown that such mutants can be employed to examine phage biology and assign functions to putative genes.

Erythromycin

Analysis of the collar-whisker structure of temperate lactococcal bacteriophage TP901-1

Article Abstract:

Study was conducted to test the hypothesis that neck passage structure (NPS) was involved in the infection of phage TP901-1 by analysis of an NPS mutant. It was determined to form a collar-whisker complex but was shown as nonessential for infection, phage assembly and stability.

Science & research, Genetic aspects, Bacterial proteins, Structure, Lactococcus

Identification of a replication protein and repeats essential for DNA replication of the temperate lactococcal bacteriophage TP901-1

Article Abstract:

The gene orf13 of the lactococcal bacteriophage TP901-1 codes a replication protein that is critical for DNA replication. Replication appears to originate at repeats located within the gene.

DNA