Flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose

Article Abstract:

Results demonstrate that in high-spirochete communities a dramatic shift in the relative abundance of fermentative bacteria is seen upon glucose shock whereas in low-spirochete communities perturbation effects are smaller. Data point out that community stability and functional stability are inversely correlated.

author: Tiedje, James M., Raskin, Lutgarde, Hashsham, Syed A., Criddle, Craig S., Fernandez, Ana S., Dollhopf, Sherry L., Glagoleva, Olga, Dazzo, Frank B., Hickey, Robert F.
United States, Analysis, Physiological aspects, Biotic communities, Methanobacteriaceae, Methanogens, Bioreactors, Glucose

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Corresponding between community structure and function during succession in phenol- and phenol-plus-trichloroethene-fed sequencing batch reactors

Article Abstract:

The effects of long-term trichloroethene (TCE) application on community structure, succession and reactor performance, by using sequencing batch reactors (SBRs) were determined. Periodic changes were observed in the SBR that received phenol only and these changes were not seen in the phenol-plus-TCE fed reactor.

author: Tiedje, James M., Criddle, Craig S., Ayala-del-Rio, Hector L., Callister, Stephen J.
All Other Basic Organic Chemical Manufacturing, Industrial Organic Chemicals, Cyclic crudes and intermediates, Cyclic Crude and Intermediate Manufacturing, Phenol, Trichloroethane, Phenol (Compound), Structure, Chemical properties, Trichloroethane (1,1,1-trichloroethane)

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Influence of dangling ends and surface-proximal tails of targets on probe-target duplex formation in 16S rRNA gene-based diagnostic arrays

Article Abstract:

The effect of danglings ends and surface-promixal tails of gene targets on probe-target duplex formation is evaluated by using an oligonucleotide microarray containing 18-mer probes corresponding to the 16S rRNA genes of 10 waterborne pathogens and a number of synthetic and PCR-applied gene targets. The study has highlighted the requirement of a careful target preparation and evaluation of signal intensities for diagnostic arrays by using 16S rRNA and other gene targets because of the potential for target interaction with a complex background.

author: Tiedje, James M., Cole, James R., Hashsham, Syed A., Gulari, Erdogan, Stedtfeld, Robert D., Wick, Lukas M., Baushke, Samuel W., Tourlousse, Dieter M., Herzog, Amanda B., Yongmei Xia, Rouillard, Jean Marie, Klappenbach, Joel A.
Science & research, Research, Genetic aspects, Ribosomal RNA, Pathogenic microorganisms, Report

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