Emergence of distinct genotypes of Cryptosporidium parvum in structured host populations

Article Abstract:

Cryptosporidium parvum is an apicomplexan parasite that infects humans and ruminants which is isolated from cattle in northeastern Turkey and in Isreal and was genotyped using multiple polymorphic genetic makers, structure. The results explain the frequent detection of host-specific genotypes with unknown taxonomic status in surface water and existence of geographically restricted Cryptosporidium parvum in humans.

author: Widmer, Giovanni, Tanriverdi, Sultan, Markovics, Alex, Arslan, M.Ozkan, Itik, Aysel, Shkap, Varda
Turkey, Genetic aspects, Genetic markers, Host-parasite relationships

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Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples

Article Abstract:

Research was conducted to evaluate the effect of immunomagnetic separation (IMS) on oocyst infectivity by means of in vitro cell culture assay combined with mRNA extraction and reverse transcriptase PCR. Two IMS kits for Cryptosporidium parvum were compared for recovery of oocysts from environmental samples. Results provide evidence that oocysts that were recovered by IMS retained their infectivity.

author: Johnson, Anne, Stewart, Mic H., Leon, Ricardo De, Rochelle, Paul A., Wolfe, Roy L.
Usage, Coccidia, Oocyte donation, Microbiological assay, Waterborne infections

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UV inactivation of Cryptosporidum hominis as measured in cell culture

Article Abstract:

A study utilized cell culture-based methods to demonstrate that Cryptosporidium hominis oocysts displayed similar levels of infectivity and had the same sensitivity to UV light as Cryptosporidium parvum. Results revealed that the water industry could be confident about extrapolating Crytosporidium parvum UV disinfection data to Crytosporidium hominisoocysts.

author: Widmer, Giovanni, Johnson, Anne M., Linden, Karl, Ciociola, Kristina M., Leon, Ricardo De, Rochelle, Paul A.
United States, Cell culture

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subjects list: Research, Cryptosporidium, Oocytes