Engineering the monomer composition of polyhydroxyalkanoates synthesized in Saccharomyces cerevisiae

Article Abstract:

Polyhydroxyalkanoates (PHA) polymers composed of monomers ranging from 4 to 14 carbon atoms in either the cytosol or the peroxisome of Saccharomyces cerevisiae by harnessing intermediates of fatty acid metabolism are synthesized. It was observed that the ability of the PHA polymers permitted the rational design and synthesis of polymers with desired material properties.

Analysis, Usage, Microbiological synthesis, Gas chromatography, Chemical properties, Polyhydroxyalkanoates

Construction of a library of human glycosyltransferases immobilized in the cell wall of Saccharomyces cerevisiae

Article Abstract:

A study expressed fifty-one human glycosyltransferases in Saccharomyces cerevisiae as immobilized enzymes and are assayed for enzymatic activities. Results reveal that more than 75% of expressed recombinant glycosyltransferases retained their enzymatic activities in the yeast cell wall fraction and would be used as a human glycosyltransferase library.

Glycosylation

Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress

Article Abstract:

The unsaturated fatty acids present in the cell membrane of Saccharomyces cerevisiae render it the ability to adapt to the external conditions. The increased fluidity of the membrane lipid bilayer of these cells increases the stress resistance to freezing.

Bacterial cell walls, Membrane lipids