Flow sorting of microorganisms for molecular analysis

Article Abstract:

Flow cytometry criteria were used to sort particular cell types from water, sediment and activated sludge samples to determine if these sorted cells will be useful in molecular analysis. This method will provide investigators with a technique to study less abundant but conspicuous bacterial populations. Further, the method will serve as a screening tool to remove contaminants even in abundant populations.

Usage, Flow cytometry, Cell separation

Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification

Article Abstract:

In-situ hybridization has found increasing use in nucleic acid probes in studying the specific sequences of whole-fixed bacterial cells. The use of fluorescein-tyramide as a substrate for oligonucleotides labeled with horseradish peroxidase can improve the in situ hybridization of bacterial cells. Flow cytometry measurements show that the hybridized cells have a 10- to 20-fold signal amplifications.

Research, Innovations, In situ hybridization, Nucleic acid probes, Bacterial genetics

Diversity and abundance of aerobic and anaerobic methane oxidizers at the Haakon Mosby mud volcano, Barents Sea

Article Abstract:

The microbial diversity and community structure in methane rich sediments of the Haakon Mosby Mud Volcano are investigated by comparative sequence analysis of 16S rRNA genes and fluorescence in situ hybridization. Result shows the presence of both aerobic and anaerobic methane oxidizers are found in lower abundances.

Arctic, Analysis, Physiological aspects, Environmental aspects, Genetic aspects, Polymerase chain reaction, Bacteria, Aerobic, Aerobic bacteria, DNA synthesis, Submarine volcanoes