Article Abstract:
The partitioning of 14C from different labeled anthracenes under a variety of conditions during microbial degradation in soil and compost mixtures was traced to investigate the mechanisms of carbon partitioning and residue formation. Results revealed high levels of mineralization that were caused by the addition of compost and its specific microflora, as well as complete transformation of the parent compound anthracene. Results revealed further that residues may arise from CO2 in the soil in amounts typical for anthracene biodegradation, with the extent of residue formation dependent on the metabolic capacity of the soil microflora and the characteristics of the soil.
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Article Abstract:
A highly heat-stable proteinase from the extremely thermophilic archaeon Thermococcus stetteri was purified utilizing the enzyme's sodium dodecyl sulfate resistance. Characterized as a serine protease with limited substrate specificity and high stereospecificity, the enzyme also acted as an acyl transferase. Thermally stable optimal activity was at 85 degrees Celsius and pH 8.5 to 9. Some characteristics of the proteinase may be explained by the tight packing and inflexibility indicated by its fluorescence spectra.
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Article Abstract:
Results reveal that naphthalene degradation pathway mediated by the thermophilic Bacillus thermoleovorans deviates from the established pathways. This finding is supported by the identification of specific intermediates that differe from those found in the mesophilic bacteria.
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