Frequency and distribution of tetracycline resistance genes in genetically diverse, nonselected, and nonclinical Escherichia coli strains isolated from diverse human and animal sources

Article Abstract:

Resistance towards tetracycline among the natural and unselected populations of Escherichia coli from animals and humans was examined so that the distribution of the resistance genes in the environment and an insight into the selection pressures involved with the use of antibiotics could be determined. The results suggested the presence of the selection pressures in the various hosts.

Analysis, Antibiotics, Escherichia coli

Purification and characterization of TrzF: Biuret hydrolysis by allophanate hydrolase supports growth

Article Abstract:

TrzF, the allophanate hydrolase from Enterobacter cloacae strain 99, was cloned, overexpressed in the presence of a chaperone protein, and purified to homogenity. TrzF showed time-dependent inhibition by phenyl phosphorodiamidate and is a member of the amidase signature protein family.

Enzymes, Enterobacter, Enterobacteriaceae, Hydrolysis, Hydrolases

Tetracycline resistance mediated by tet(W), tet(M), and tet(O) genes of Bifidobacterium isolates from humans

Article Abstract:

MICs of tetracyclines are determined for human Bifidobacterium isolates and three environmental strains. Chromosomal DNA hybridization has indicated that there is one chromosomal copy of tet(W) and/or tet(M).

Environmental aspects, Chromosome mapping, Bifidobacterium, Bifidobacteria