Functional analysis of upstream regulating regions for the Yarrowia lipolytica XPR2 promoter

Article Abstract:

A study was conducted to analyze the transcriptional function of each upstream activating sequence (UAS) to the activity of the XPR2 gene from Yarrowia lipolytica and the contribution of the putative PaC/Rim101p targets to its regulation. Experimental results indicated that the UAS were characterized by several interacting elements. Findings also showed that the UAS were major determinants of promoter activities.

author: Gaillardin, Claude, Madzak, Catherine, Blanchin-Roland, Sylvie, Cordero Otero, Ricardo R.
Gene expression, Promoters (Genetics), Genetic transcription, Transcription (Genetics)

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Autocloning and amplification of LIP2 in Yarrowia lipolytica

Article Abstract:

Researchers describe an autocloning method that produces multiple copies of the gene for extracellular lipase in Yarrowia lipolytica. These strains could produce 40 times more lipase per liter than strains containing only one copy of the gene. This makes them a promising source of lipase for industrial use, waste treatment, and pancreatic insufficiency treatment.

author: Nicaud, Jean-Marc, Pignede, Georges, Wang, Hui-Jie, Fudalej, Franck, Seman, Michel, Gaillardin, Claude
Microbial genetic engineering, Lipase, Industrial microorganisms

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Molecular characterization of Yarrowia lipolytica and Candida zeylanoides isolated from poultry

Article Abstract:

Research describes genetic analyses of two yeast strains isolated from poultry meat by various molecular characterization techniques. Data are useful in assessing the biodiversity of the yeast strains and their role in the spoilage.

author: Deak, T., Chen, J., Beuchat, L.R.
United States, Hungary, Methods, Analysis, DNA, Poultry, Poultry (Animals), Poultry (Meat), Molecular biology, Yeast, Yeast (Food product), Contamination, Molecular microbiology

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subjects list: Research, Genetic aspects