Functionally distinct, sequence-specific replicator and origin elements are required for Drosophila chorion gene amplification

Article Abstract:

Sequence-specific, functionally distinct replicator and origin elements have been found to be necessary for Drosophila chorion, or eggshell, gene amplification. To study sequence requirements for amplification, researchers used a vector in which transgenic constructs are shielded from chromosomal position effects the suppressor Hairy-wing protein binding site, as flanking insulator elements . A 320-bp amplification control element (ACE3) appears to be a replicator. It seems to support and extend the replicator model for the organization of metazoan chromosomal replicons.

Physiological aspects, Carrier proteins, Transport proteins, Chorion

ORC binding, gene amplification, and the nature of metazoan replication origins

Article Abstract:

Origin recognition complex (ORC) binding specificity, gene amplification, and metazoan replication origins are discussed. Metazoan eukaryotes may use novel mechanisms to control DNA replication, but this is the subject of controversy. Replication in budding yeast and in metazoans have differences in the nature of cis-acting elements necessary for origin initiation.

Metazoa, Metazoans, Saccharomyces, Xenopus

Drosophila ORC specifically binds to ACE3, an origin of DNA replication control element

Article Abstract:

ACE3, an origin of DNA replication control element and Drosophila origin recognition complex (DmORC) are discussed. Through use of in vivo and in vitro approaches, it has been shown that ACE3 and AER-d are DNA-binding sites for DmORC. Likely DmORC DNA-binding specificity controls origin selection. Identifying DmORC-binding sites in vivo probably will be powerful in identifying other Drosophila origins.

Cellular control mechanisms, Cell regulation