Genomic analysis of Pediococcus starter cultures used to control Listeria monocytogenes in turkey summer sausage

Article Abstract:

The role of pediocin production in Pediococcus starter cultures used to control Listeria monocytogenes in turkey summer sausage was determined. The experiments assessed the efficiency of non-pediocin-producing mutants versus the pediocin-producing cultures. The results showed that pediocin-producing starter cultures are associated with more dramatic reduction in counts of L. monocytogenes during fermentation. Further analysis showed that P. acidilactici starter cultures and pediocin-encoding plasmids are genetically related. Not all of the commercially available starter cultures encode pediocin or are equally effective in controlling L. monocytogenes.

author: Harsono, Kartika D., Luchansky, John B., Glass, Kathleen A., Degnan, Alan J., Faith, Nancy G., Cauvin, Beatrice, Baccus-Taylor, Gail, Arihara, Keizo, Bater, Bilig, Maurer, Arthur J., Cassens, Robert G.
Genetic aspects, Control, Sausage, Sausages, Bacteriology Cultures and culture media

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Relatedness of Listeria monocytogenes isolates recovered from selected ready-to-eat foods and listeriosis patients in the United States

Article Abstract:

Pulsed-field gel electrophoresis and serotyping are performed for 544 isolates of Listeria monocytogenes, including 502 isolated recovered from contaminated samples from many retail ready-to-eat (RTE) food products and 42 isolates recovered from human cases of listeriosis. The results help in defining the distribution and relatedness of isolates found in RTE foods in comparison with isolates that cause listeriosis.

author: Luchansky, John B., Wallace, F. Morgan, Scott, Virginia N., Yuhuan Chen, Gilbreth, Stefanie Evans, Call, Jeff E.
Food Manufacturing, Science & research, FOOD AND KINDRED PRODUCTS, Prepared Perishable Foods, Perishable Prepared Food Manufacturing, Convenience Foods, Listeriosis

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Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis

Article Abstract:

The foodborne Listeria species were analysed using the pulsed- field gel electrophoresis (PFGE) technique differentiating Listeria monocytogenes from other listeria species. It enabled differentiation of serologically and phenotypically similar listeriae due to the sensitivity and specificity of homogeneous electric pulsed-field analysis.

author: Howard, Patricia J., Harsono, Kartika D., Luchansky, John B.
Usage, Foodborne diseases, Listeria, Microbial differentiation

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subjects list: Health aspects, Listeria monocytogenes, Research, Methods, Gel electrophoresis