Genotypic characterization of Bradyrhizobium strains nodulating small senegalese legumes by 16S-23S rRNA intergenic gene spacers and amplified fragment length polymorphism fingerprint analyses

Article Abstract:

Genotypic analysis indicate that 64 strains of Bradyrhizobium are diverse and form subsets of groups based on the type of genetic analysis technique employed. Groupings by the amplified fragment length analysis, intergenic spacer PCR and restriction fragment length polymorphism, and 16S amplified ribosomal DNA restriction analysis methods were similar.

author: Doignon-Bourcier, Florence, Willems, Anne, Coopman, Renata, Laguerre, Gisele, Gillis, Monique, Lajudie, Philippe de
United States, Belgium, Statistical Data Included, Methods, Biological diversity, Biodiversity, Molecular biology

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Compatibility of rhizobial genotypes within natural populations of Rhizobium leguminosarum biovar viciae for nodulation of host legumes

Article Abstract:

Research indicates that host plants exert influence on the genetic diversity of Rhizobium leguminosarum bv. viciae populations as well as host specificity differences within Vicieae cross-inoculation group. Data show that genotypes of nodule populations are not necessarily competitive for nodule occupancy, suggesting both genomic composition and genomic background influence competitiveness for nodulation.

author: Laguerre, Gisele, Louvrier, Philippe, Allard, Marie-Reine, amarger, Noelle
Science & research, Soil microbiology

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Photosynthetic bradyrhizobia from Aeschynomene spp. are specific to stem-nodulated species and form a separate 16S ribosomal DNA restriction fragment length polymorphism group

Article Abstract:

Nine bacterial isolates were obtained from root or collar nodules of the non-stem-nodulated Aeschynomene species and 69 root or stem nodule isolates from the stem-nodulated Aeschynoemene species from various places in Senegal. These isolates, together with 45 previous isolates from various Aeschynomene species, were investigated for host-specific nodulation within the genus Aeschynomene. Results showed that photosynthetic Aeschynomene isolates share the ability to nodulate particular stem-nodulated species and form a separate subbranch on the Bradyrhizobium rRNA lineage, distinct from B. japonicum and B. elkanii.

author: Giraud, Eric, Willems, Anne, Gillis, Monique, Lajudie, Philippe de, Lorquin, Jean, Dreyfus, Bernard, Hoste, Bart, Molouba, Flore, Masson-Boivin, Catherine
Bacteria, Photosynthetic, Photosynthetic bacteria, Restriction enzymes, DNA, DNA restriction enzymes

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subjects list: Analysis, France, Genetic aspects, Rhizobium, Genomes, Microbial populations, Research