Genotyping male-specific RNA coliphages by hybridization with oligonucleotide probes

Article Abstract:

Genotyping of F(super +) RNA coliphages by nucleic acid hybridization with nonradioactive oliginucleotide probes is an effective and more economical method to group them. Genotyping of F(super +) RNA coliphages is practically viable for field samples. Serotyping of F(super +) RNA coliphages is necessary to distinguish between human fecal contamination and that of animal to determine the risk of infection from coliphage from different sources. Results obtained from genotyping compare very well with those from serotyping.

Environmental aspects, Water pollution, Phenotype, Phenotypes, Water quality bioassay

Molecular detection and genotyping of male-specific coliphages by reverse transcription-PCR and reverse line blot hybridization

Article Abstract:

A reverse line blot hybridization (RLB) assay was developed for the simultaneous detection and genotyping of FRNA and FDNA coliphages. It is concluded that RLB method is a fast, reproducible, cheap, and easy to perform with a high throughput of samples and this makes RLB an ideal candidate to become a standardized method for the detection of FRNA and DNA coliphages as source specific indicators of fecal contamination in environmental waters and shellfish.

Shellfish, Shellfish Fishing, Hybridization

Evaluation of F+ RNA and DNA coliphages as source-specific indicators of fecal contamination in surface waters

Article Abstract:

The presence and proportions of the different subgroups of male specific (F+) coliphages in a variety of fecal wastes and surface waters with well-defined potential waste impact is examined. Monitoring of F+ coliphage groups can indicate the presence and major sources of microbial inputs to surface waters, but environmental effects on the relative occurrence of different groups need to be considered.

Microbiological research