Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification

Article Abstract:

Amplification of 16S rRNA from mycoplasmal organisms using the polymerase chain reaction allowed the identification of species- and genus-specific sequences suitable for identifying the organisms. The species-specific sequences hybridized only with the corresponding species. The genus-specific sequence was highly specific for mycoplasma but also cross-reacted with some related organisms. The V2, V3, V5 and V7 regions of the 16S rRNA were used to develop the species-specific primers, while the genus-specific primer was obtained from nucleotides 1029 to 1055.

Nucleotide sequence, Base sequence

Application of a Mycoplasma group-specific PCR for monitoring decontamination of Mycoplasma-infected Chlamydia sp. strains

Article Abstract:

Incubation with Triton X-100 is useful in the decontamination of the laboratory strains of Chlamydia pneumoniae, C. pecorum and C. trachomatis, infected with Mycoplasma. A Mycoplasma group-specific PCR technique detects the contamination of cell lines. The contamination with Mycoplasma spp. changes the experiment parameters of Chlamydia strains and necessitates a Mycoplasma-free material. Antibodies cannot be used against mycoplasma as they suppress the Chlamydia cells.

Observations, Cell lines, Microbial contamination, Chlamydia

Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction

Article Abstract:

The detection of Campylobacter spp. in chicken products usinga combination of short enrichment culturee and polymerase chain reaction (PCR) is discussed. This involved the development of a specific PCR and probe hybridization assay based on the 16S rRNA gene sequence. A comparison of the method with conventional detection techniques yielded the same results. However, the results from the PCR-culture assay was obtained faster.

Campylobacter