Group-specific small-subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems

Article Abstract:

The characteristics of filamentous foaming in full-scale activated sludge were analyzed by utilizing group-, genus- and species-specific small subunit (SSU) ribosomal ribonucleic acid (rRNA)-targeted oligonucleotide probes. The hybridization probes were designed based on the phylogeny of the mycolic acid-containing actinomycetes inferred by SSU rRNA sequence analysis from the Ribosomal Database Project. The nested set of oligonucleotide probes for filamentous bacteria was efficiently utilized for the quantification of filamentous microbial populations in activated sludge.

Methods, Analysis, Design and construction, Nucleic acid hybridization, Identification and classification, Nucleic acid probes, Ribosomal RNA, Sewage sludge

Quantification of Gordona amarae strains in foaming activated sludge and anaerobic digester systems with oligonucleotide hybridization probes

Article Abstract:

An oligonucleotide probe was developed to examine the role of interstrain heterogeneity in the small subunit ribosomal DNA sequences of Gordona amarae in the different responses of G. amarae strains with previous species-specific probes. G. amarae implicated in foaming activated sludge and anaerobic digester system was identified and characterized using the probe. The specificities of the probes were investigated by the hybridization of various RNAs obtained from target and nontarget bacteria.

RNA, Nucleotides, Sludge, Ribosomal proteins

Quantification of syntrophic fatty acid-beta-oxidizing bacteria in a mesophilic biogas reactor by oligonucleotide probe hybridization

Article Abstract:

Research is conducted on the oligonucleotide probe detection of fatty acid-beta-oxidizing bacteria to characterize the biogas reactor microbial community. Hybridization results are included.

International, Usage, Genetic aspects, Fatty acids, Bioreactors, Molecular microbiology, Hybridization