Article Abstract:
Two genetically manipulated forms of Lactobacillus plantarum were studied for their growth and persistence in grass silage. Both recombinants contained a gene for erythromycin resistance called pSA3. One had pSA3 incorporated in its chromosome and the other had a pSA3 derivative maintained in a plasmid. Both strains proliferated in silos to a similar extent as the parent strain, which was used as a control. All of the L. plantarum strains eventually dominated the silage microflora. The presence of this extra genetic material therefore did not affect growth and survival.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
A study has confirmed that Pediococcus acidilactici lactic acid 3 and 35 are effective silage inoculants that these substances can be more effective in improving silage quality than Pediococcus pentosaceus under high temperature conditions. Experiments were conducted to isolate Pediococcus species from forage crops for characterization and application to silage preparation. These isolates revealed three different strains of the bacteria and used as additives to alfalfa and Italian ryegrass silage preparation at two temperatures, namely 25 deg and 48 deg Celcius.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
Homology between the smallest, cryptic plasmid, pA1, and pE194 was determined for Lactobacillus plantarum by plus-origin-specific-probes. Three putative reading frames capable of encoding proteins were identified by sequence analysis. In vitro transcription translation assays were used to characterize two of these proteins. The replication of pA1 by rolling-circle mode of replication was demonstrated.
User Contributions:
Comment about this article or add new information about this topic: