Identification of pyruvate carboxylase genes in Pseudomonas aeruginosa PAO1 and development of a P. aeruginosa-based overexpression system for [alpha.sub.4]- and [alpha.sub.4][beta.sub.4] -type pyruvate carboxylases

Article Abstract:

An [alpha.sub.4]- type and an [alpha.sub.4][beta.sub.4] -type pyruvate carboxylase (PYC), for which Escherichia coli is not a suitable host, that can be overexpressed recombinantly in Pseudomonas aeruginosa, is presented. Pseudomonas aeruginosa type strain that allowed the expression of recombinant PYCs in the absence of the native enzyme, is developed which can be transformed genetically and lysed for cell extract preparation easily.

Indonesia, Analysis, Observations, Growth, Gene expression, X-ray crystallography, Company growth, Pyruvate carboxylase

Effect of superoxide dismutase gene inactivation on virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori

Article Abstract:

The role of superoxide dismutase (SOD) in virulence against the silkwork, Bombyx mori explored by generating mutants of Pseudomans aeruginosa showed that full virulence of Pseudomans aeruginosa against Bombyx mori and Fe-SOD is more important than Mn-SOD in the infection process.

Autotransporter-encoding sequences are phylogenetically distributed among Escherichia coli clinical isolates and references strains

Article Abstract:

The development and validation of a multiplex PCR assay to screen autotransporter genes in Escherichia coli showed that the autotransporter gene profiles might be useful for comparative analysis of Escherichia coli strains from clinical, food, and environmental sources.

Escherichia coli