Article Abstract:
Toluene degradation has been linked with specific microbial populations in soil. Traditional culture-based approaches and phospholipid fatty acid (PLFA) and C-PLFA methods not affected by biases associated with laboratory cultivation were used to identify the indigenous populations responsible for disappearance of toluene in Yolo silt loam, a surface soil. PLFA analysis of a soil microbial community was coupled with (super.13)C isotope tracer analyses to measure the community's response to addition of 35 micrograms of (super.13)toluene/ml of soil solution. Results showed coupling the methods is effective for distinguishing a specific microbial population involved in metabolism of a labeled substrate in complex environments such as soil.
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Article Abstract:
The isolation of saprophytic basidiomycetes from soil involves selective inhibitors, an indicator substrate and soil particle washing. The washed organic particles are plated on a medium containing lignin, guaiacol and benomyl to reduce mold growth and allow detection of basidiomycetes producing laccase. The method is useful for the investigation of biodiversity of soil basidiomycetes and gives organisms for bioremediation of soils contaminated with pesticides.
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Article Abstract:
The communities of soil basidiomycetes in agroecosystems that differ in tillage history at the Kellog Biological Station Long-Term Ecological Research site near Battle Creek, Michigan are analyzed. Results reveal that only 24 of 241 species had 99% or greater sequence similarity to named reference sequences in GenBank, and several clades with multiple 'species' could not be identified at the genus level by phylogenetic comparisons with named sequences.
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