Lipoxygenase inhibitors shift the yeast/mycelium dimorphism in Ceratocystis ulmi

Article Abstract:

Gossypol, nordihydroguaiaretic acid and propylgallate, known lipoxygenase inhibitors, converted the mycelial form of Ceratocystis ulmi into the yeast form. Cyclooxygenase and thromboxane synthetase inhibitors did not convert mycelia into yeasts. C. ulmi conidiospores were found to be rich in oleic, linoleic and linolenic acids and devoid of arachidonic acid. Linoleic and linolenic acids are presumed to be the substrates for fungal lipoxygenase. These results indicate that fungal lipoxygenase may be a potential target for the development of antifungal antibiotics.

Fungi, Phytopathogenic, Phytopathogenic fungi, Enzyme inhibitors

The Entner-Doudoroff pathway in Escherichia coli is induced for oxidative glucose metabolism via pyrroloqiuinoline quinone-dependent glucose dehydrogenase

Article Abstract:

Evidence that oxidation of glucose to gluconate in the periplasm is enabled by Entner-Doudoroff enzymes is presented. The study also establishes the role of limiting phosphate in regulating the Entner-Doudoroff pathway. Enzyme assays and RNA isolations were conducted on Escherichia coli cells, a low phosphate concentration. It was found that the use of the Etner-Doudoroff pathway is promoted by E. coli by providing access of periplasmic pyrroloquinoline quinoneinto the periplasm.

Methods, Escherichia coli, Glucose metabolism

Survey of extreme solvent tolerance in gram-positive cocci: Membrane fatty acid changes in Staphylococcus haemolyticus grown in toluene

Article Abstract:

The unique ecological niche of oil fly larval guts is exploited to isolate a strain of Staphylococcus haemolyticus, which may be the most solvent-tolerant gram-positive bacterium. Results reveal that six strains of Staphylococcus aureus and five strains of Staphylococcus epidermis, which were not obtained by solvent selection, exhibited exceptional solvent bacteria.

Science & research, Petrochemical Manufacturing, Toluene, Gram-positive bacteria, Staphylococcus, Genetic research