Localization and solubilization of the iron(III) reductase of Geobacter sulfurreducens

Article Abstract:

Research was conducted to examine the localization and solubilization of the iron(III) reductase of Geobacter sulfurreducens. Reductase activity was measured with the electron donor NADH and the artificial electron donor horse heart cytochrome c. Results derived from the isolation of the outer membrane and by treating whole cells with a high KCl concentration to solubilize the iron(III) reductase suggest that the iron(III) reductase of G. sulfurreducens is a peripheral protein of the outer membrane. Findings also indicate that a c-type cytochrome is involved in iron(III) reduction.

Bacteria, Oxidation-reduction reaction, Oxidation-reduction reactions, Cytochrome c, Electron donor-acceptor complexes, Fractionation, Fractional distillation

Characterization of the corrinoid iron-sulfur protein tetrachloroethene reductive dehalogenase of Dehalobacter restrictus

Article Abstract:

The authors have investigated the membrane-bound tetrachloroethene reductive dehalogenase which is the terminal component of Dehalobacter restrictus respiratory chain. The aim of their research has been the purification and biochemical characterization of the bacterium and its strains, and the cloning and sequencing of bacteria's genes.

Netherlands, Science & research, All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Perchloroethylene, Genetic aspects, Microbiology, Cloning, Iron compounds, Sulfur bacteria, Gram-positive bacteria, Bacterial proteins, Microbial populations, Sulphur bacteria, Microbial ecology

Evidence for the existence of psychrophilic methanogenic communities in anoxic sediments of deep lakes

Article Abstract:

Results show that profundal lake sediments, which are low-temperature environments, contain low-temperature-adapted psychrophilic methanogenic communities although the methane production by such bacteria can only be demonstrated at mesophilic or thermophilic temperature range.

Russia, Analysis, Methane, Microbial metabolism, Thermal properties, Methanobacteriaceae, Methanogens, Lake sediments