Article Abstract:
Screening experiments concerned with the bioconversion of oleic acid were described. Resting cells of Saccharomyces cerevisiae hydrated oleic acid into 10-hydroxyoctadecanoic acid, while Mycobacterium fortuitum, Nocardia sp. and Nocardia aurantia all converted oleic acid into 10-oxo-octadecanoic acid. The Baeyer-Villiger chemical oxidation of 10-oxo-octadecanoic acid confirmed the position of oxygenation in the fatty acid ketone. Furthermore, a simple nuclear magnetic resonance spectral technique measured the stereochemical purity of hydroxy fatty acids.
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Article Abstract:
The ferulic acid degradative pathway followed by Pseudomonas fluorescens and Saccharomyces cerevisiae were investigated. Metabolites were isolated by solid-phase extraction and analyzed by thin-layer chromatography and high performance liquid chromatography. Based on the analysis, a new mechanism for decarboxylation of ferulic acid is proposed. The proposed mechanism is nonoxidative, and was carried out by both organisms in media containing deuterium dioxide.
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Article Abstract:
The stereochemical purities of the hydroxy fatty acids was ascertained by a method based on the H-nuclear magnetic resonance spectral analysis of diastereomeric S-(+)-O-acetylmandelate esters of hydroxysearates. This involves the description of microbial hydrations stereochemistries of oleic acids to six cultures of microorganisms. The capability of the NMR spectral method for determining absolute configurations and stereochemical purities was demonstrated.
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