Microbial proline 4-hydroxylase screening and gene cloning

Article Abstract:

The partial purification, proline 4-hydroxylase screening, cloning and expression of the proline 4-hydroxylase gene in Escherichia coli were conducted. The proline 4-hydroxylase studied is classified as a 2-oxoglutarate-dependent dioxygenase since it requires 2-oxoglutarate and ferrous ion for a reaction. It catalyzed highly regio- and stereospecific hydroxylation of L-proline. The rigidity of the reaction proves the usefulness of the enzyme as a biocatalyst for the production of trans-4-hydroxy-L-proline from L-proline.

Microbial enzymes, Cloning, Hydroxylases

Construction of a reporter vector for the analysis of Bifidobacterium longum promoters

Article Abstract:

A versatile reporter plasmid based on a Bifidobacterium longum cryptic plasmid and the Escherichia coli gusA gene is constructed to initiate studies on promoter activities in Bifidobacterium langum and to independently confirm transcriptional data generated by microarray experiments. The resulting plasmid, pMDY23 is tested using three B. longum promoters.

Bifidobacterium, Bifidobacteria

Improvement of NADPH-dependent bioconversion by transcriptome-based molecular breeding

Article Abstract:

The development of enhanced model system for NADPH-dependent bioconversion from glucose metabolism in Escherichia coli is described. Transcriptome data from a xylitol-producing recombinant Escherichia coli are used to increase its productivity.

Xylitol, Chemical properties, NADP (Coenzyme), Nicotinamide adenine dinucleotide phosphate