A RACHITT for our toolbox

Article Abstract:

The authors used RACHITT (random chimeragenesis on transient templates) to create a hybrid dibenzothiophene monooxygenase that is more efficient at biodesulfurization of fossil fuels than parent genes to show the effectiveness of this method of gene shuffling. The article also refers to "DNA shuffling method of regenerating highly recombined genes and evolved enzymes" of this issue (p. 354-359).

PETROLEUM AND COAL PRODUCTS, Petroleum & Energy Products, Petroleum and Coal Products Manufacturing

Sequence-activity relationships guide directed evolution

Article Abstract:

A combined computational and experimental method, which incorporates a protein sequence-activity relationship (ProSAR), is used for evolving proteins with novel properties. The ProSAR approach is used to analyze the effects of multiple mutations and also identify beneficial mutations.

General services, Research, Methods, Company systems management, Information management, Protein engineering, Molecular evolution

Production of calves from G1 fibroblasts

Article Abstract:

Researchers in Massachusetts determined that cloning calves from fibroblasts using a method called "shake-off" was more efficient than the serum starvation method.

Livestock, Except Dairy and Poultry, Cattle Ranching and Farming, Massachusetts, Beef Cattle & Calves, Beef cattle